Capture Antibody: 0.5?mg/mL of rabbit anti-Human Galectin-1 monoclonal antibody (in PBS,?pH 7.4). Dilute to a working concentration of 2?μg/mL in PBS before coating.
Detection Antibody: 0.2?mg/mL of rabbit anti-Human Galectin-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50?% HRP-Protector,?pH 7.4, store at 4?°C). Dilute to working concentration of 0.5?μg/mL in detection antibody dilution buffer before use.
Standard: Each vial contains 870?ng of recombinant Human Galectin-1. Reconstitute with 1?mL detection antibody dilution buffer. After reconstitution, store at -20?°C to -80?°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 20000 pg/mL is recommended.
Sensitivity: The minimum detectable dose of Human Galectin-1 was determined to be approximately 312.5 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.